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Dice rooms have different effects depending on the number of pips. When you step on the die in the middle of the room, it activates the effect. You either got a 1 or a 6 pip room, which rerolls all your items.
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The screen will shake and the room's effect will resolve. Dice rooms can be accessed through the use of two keys, and contain one of the 6 dice faces. Each room can only be used once, and each face has a different effect 1 pip rerolls isaac's currently held items, similarly to the d4 If i'm checking dice rooms, i'm pretty much exclusively looking for a 5 room.
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5 is always helpful unless you're trying to play fast (in which case you're probably not checking dice rooms), vs all the others being either highly situational or just straight up bad. Dice rooms are special rooms that can randomize many elements of the run. Dice rooms require two keys to be opened, unlike most other special rooms. For a dice room to spawn, two things must happen; First, the game must decide that a sacrifice room will spawn that floor.
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